To study various kinetic constants of catalase, cytochrome P450 and inducible nitric oxide synthase in response to extremely low frequency magnetic field exposure in two human cell lines.
In order to evaluate the effect of the magnetic field exposure on the modulation of cellular responses to an inflammatory stimulus, both cell lines were treated with lipopolysaccharide (LPS).
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
continuous for up to 25 h
|
cells were treated in the following four groups: i) control ii) Escherichia coli (LPS) iii) exposure to EMF iv) LPS + exposure to EMF
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | continuous for up to 25 h |
| Exposure source | |
|---|---|
| Setup | 22 cm long solenoid with a radius of 6 cm and 160 turns of copper wire with a diameter of 1.25 x 10-5 cm; field homogeneity in the solenoid's center = 98 %; cells positioned on a grid in the center of the solenoid; solenoid placed inside an incubator with 5 % CO2 and a constant temperature of 37 ± 0.3°C |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 1 mT | effective value | measured | - | - |
The results are not summarized by the authors and the following extracts are performed by EMF-Portal editor:
Catalase activity in HaCaT cells: most of the parameters investigated were significantly increased by LPS stimulation and to a lesser extent by magnetic field exposure, whereas co-exposure (LPS + magnetic field) showed no effect compared with the control.
Catalase activity in TPH-1 cells: magnetic field exposure showed for the most parameters investigated opposite or more pronounced effects as compared with LPS stimulation alone or co-exposure (LPS + magnetic field), e.g. magnetic field exposure significantly increased the total velocity, whereas co-exposure or LPS alone caused a decrease.
Cytochrome P450 activity in HaCaT cells: The plot of cytochrome 450 activity under magnetic field exposure did not fit into Gaussian distribution equation, so exact values for any kinetic parameter in response to the magnetic field exposure were not available, except for the minimum velocity (5-fold increase compared to the control).
Cytochrome P450 activity in TPH-1 cells: Total velocity and minimum velocity were increased following LPS treatment, magnetic field exposure and co-exposure compared with the control. "Rate of decrease", peak time and peak velocity in response to the magnetic field exposure could not be detected.
iNOS activity in HaCaT cells: most of the parameters investigated were significantly increased by LPS stimulation as well as by co-exposure (more pronounced) and to a lesser extent by magnetic field exposure.
The authors conclude that the cell lines examined showed similar trends in response to combined treatment with LPS and an extremely low frequency magnetic field but with totally different catalytic values, which indicates the distinction between neuronal and non-neuronal cells.
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