Medical/biological study (experimental study)

The effects of exposure to extremely low-frequency magnetic field and amphetamine on the reduced glutathione in the brain med./bio.

By: Jelenkovic A, Janac B, Pesic V, Jovanovic MD, Vasiljevic I, Prolic Z

Published in: Ann N Y Acad Sci 2005; 1048: 377-380

Aim of study (acc. to editor)

To study the effect of continuous exposure to an extremely low frequency magnetic field alone and combined with D-amphetamine (1.5 mg/kg) on reduced glutathione content in six brain regions of rats.

Background/further details

Amphetamine can increase the reduced glutathione content and a pro-oxidative effect of amphetamine has been reported.
Two of four groups of rats (n=6 per group) were exposed (the remaining two groups were sham exposed) and immediately after the exposure (or sham exposure), two groups (exposed and sham-exposed) were treated with D-amphetamine.

Endpoint

effects on the neurological system: oxidative metabolism in the brain

Exposure

- 50/60 Hz
- magnetic field

Exposure	Parameters
Exposure 1: 50 Hz Exposure duration: continuous for 7 day	magnetic flux density: 0.5 mT unspecified

General information

Rats were divided into four group (n=6/group), 2 groups were exposed to MF, the remaining two were sham exposed.

Exposure 1

Main characteristics

Frequency	50 Hz
Type	magnetic field
Exposure duration	continuous for 7 day

Exposure setup

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	0.5 mT	unspecified	unspecified	-	-

Exposed system:

Methods Endpoint/measurement parameters/methodology

effects on the neurological system: oxidative metabolism in different brain regions (reduced glutathione content; spectrophotometry)
cognitive/behavioral endpoints: motor activity (in the open field)

Investigated system:

crude mitochondrial fractions of cortex, striatum, basal forebrain, hippocampus, brainstem, and cerebellum
investigation on living organism

Investigated organ system:

brain/CNS

Time of investigation:

after exposure

Main outcome of study (acc. to author)

Magnetic field exposure induced a significant decrease in glutathione only in the cortex. Amphetamine alone did not change the glutathione content. When it was given after extremely low frequency electromagnetic field exposure, glutathione depletion in the cortex induced by magnetic field exposure was completely prevented. Furthermore, the glutathione content was increased in the brainstem and cerebellum of animals treated with "magnetic field exposure + amphetamine" compared to the others groups (sham exposure, exposure alone, amphetamine alone).
The data indicate that biogenic monoamines are involved in the glutathione content changes observed. The changes are not uniform in the brain regions examined.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

Ministry of Science and Environmental Protection, Serbia

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