Studientyp:
Medizinische/biologische Studie
(experimentelle Studie)
A small temperature rise may contribute towards the apparent induction by microwaves of heat-shock gene expression in the nematode Caenorhabditis Elegans
med./bio.
[Ein geringer Temperaturanstieg trägt möglicherweise zur Mikrowellen-induzierten Expression von Hitzeschock-Proteinen beim Fadenwurm Caenorhabditis Elegans bei]
Von:
Dawe AS, Smith B, Thomas DW, Greedy S, Vasic N, Gregory A, Loader B, de Pomerai DI
The TEM cell used here was identical to that described in [Daniells et al., 1998], except that it was constructed of copper rather than aluminium. It was 34 cm long and of a square cross-section, tapering from a maximum of 24 x 24 cm at the center to 1.5 x 1.5 cm at the ends. The inner septum (waveguide) was central and 27/32 of the total width, giving a 50 Ω impedance, which matched the load and cables. Power was limited by the matched load to 500 mW (27 dBm).
Aufbau
Identical loaded 24-well multiwell plates (containing 1.0 ml of K medium per well) were placed in two copper TEM cells located on the same shelf of a large incubator set at 26.0 ± 0.1 °C, one for RF and the other one for sham exposure.
In later experiments, an unmodified copper TEM cell was used for shams, while the TEM cell used for exposures was modified with the aim of reducing both power loss and consequent heating, including removal of internal polystyrene foam from beneath the septum, replacement of BNC by APC 3.5 connectors, and silver plating the copper surfaces of the cell.
Sham and microwave-exposed PC161 worms (as for Part A) were kept for 20 h in black non-fluorescent multiwell plates, which were removed at 2 or 4 h intervals (for <5 min per plate) to allow determination of GFP fluorescence levels.
Tkalec M et al.
(2013):
Oxidative and genotoxic effects of 900 MHz electromagnetic fields in the earthworm Eisenia fetida
Dawe AS et al.
(2009):
Low-intensity microwave irradiation does not substantially alter gene expression in late larval and adult Caenorhabditis elegans
Dawe AS et al.
(2008):
Continuous wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16-1 heat-shock gene expression in Caenorhabditis elegans
Copty AB et al.
(2006):
Evidence for a specific microwave radiation effect on the green fluorescent protein
de Pomerai DI et al.
(2000):
Microwave radiation induces a heat-shock response and enhances growth in the nematode Caenorhabditis elegans
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