Medical/biological Study (experimental study)

Resonance effect of microwaves on the genome conformational state of E. coli cells. med./biol.

PubMed Entry

Journal web site

Aim of study (according to author)
The influence of millimeter waves on the process of the genome conformational state (GCS) repair after E. coli cell exposure to X-rays was examined.
Background/further details:
As a test for appearance and repair of changes in a chromosomal DNA the method of the anomalous viscosity time dependencies (AVTD) in cell lysates was used.

Endpoint

• genotoxicity/mutation: genome conformational state

Exposure
General category: low level microwaves

Field characteristics	Parameters
field 1: 51.62 - 51.84 GHz exposure duration: 90 min	power flux density: 3 mW/cm² SAR: 17 mW/g (acoustic method) SAR: 22 mW/g (calorimetric method)
field 2: 41.25 - 41.5 GHz exposure duration: 10 min	power flux density: 200 µW/cm²
field 3: 51.62 - 51.84 GHz exposure duration: 15 min	power flux density: 3 mW/cm²
field 4: 41.25 - 41.5 GHz exposure duration: 5 min	power flux density: 1 µW/cm² min value
field 5: 51.78 GHz exposure duration: 30 min	power flux density: 3 mW/cm²

Exposed system:
bacterium (in vitro)
Escherichia coli/K12 (different strains)

Methods
Endpoint/Measurement parameters/Methodology

• genotoxicity/mutation: genome conformational state; anomalous viscosity time dependence (AVTD; relative viscosity)
• others: suppression of radiation-induced genome conformational state repair

Main outcome of study (according to author)
Radiation of E. coli cells with doses of 10-50 Gy led to changes of the AVTD curve of the cell lysate. After post-irradiated cell incubation of 90-120 min (depending on the dose of radiation), an almost complete recovery of the AVTD curve took place. This means that during this period the genome conformational state of the exposed cells returned to the control level ("repair" of genome conformational state).
The "repair" of genome conformational state of bacterial cells after ionizing irradiation is highly sensitive to the resonance effect of millimeter waves (microwaves suppress the "repair"): Within the ranges of 51.62-51.84 GHz and 41.25-41.50 GHz the frequency dependence of the observed effect has a resonance nature. The power dependence of the microwave effect within the range of 0.1-200 µW/cm² has shown that a power density of 1 µW/cm² is sufficient to suppress radiation-induced repair of the genome conformational state. The effect of microwave suppression of repair does not depend on the sequence of cell exposure to X-rays and microwave radiation.
The data indicate the role of the cell genome in the resonant interaction of cells with low intensity millimeter waves.

(Study character: medical/biological study, experimental study, full/main study)

Study funded by

• not stated/none

• Belloni F et al. (2005): A suitable plane transmission line at 900 MHz rf fields for E. coli DNA...
• Belyaev IY et al. (1993): Evidence for dependence of resonant frequency of milimeter wave interaction...
• Belyaev IY et al. (1992): Chromosome DNA as a target of resonant interaction between Escherichia Coli...