Medical/biological Study (experimental study)

Study of p53 expression and post-transcriptional modifications after GSM-900 radiofrequency exposure of human amniotic cells. med./biol.

Aim of study (according to author)
The objective of this study was to investigate whether the exposure of a radiofrequency field of 900 MHz may induce the expression of the p53 protein and its activation by phosphorylation in cultured human amniotic cells.
Background/further details:
Four average specific absorption rates were used to examine, if there is a change in the expression level of p53 protein and its phosphorylation of the serine residues 15 and 37. In response to a genetic damage, this protein is activated and can induce cell cycle arrest allowing more time for DNA repair or elimination of damaged cells through apoptosis. Cells were treated with Bleomycin as a positive control.

Endpoint

• molecular biosynthesis: expression and activating of p53 protein

Exposure
General category: mobile communication system, mobile phone, GSM, PW (pulsed wave)

Field characteristics	Parameters
900 MHz pulsed (PW) exposure duration: continuous for 24 h	SAR: 4 W/kg average over time (0.25, 1, 2, 4 W/kg) SAR: 32 W/kg peak value (2, 8, 16, 32 W/kg)

Exposed system:
intact cell/cell culture (in vitro)

Methods
Endpoint/Measurement parameters/Methodology

• molecular biosynthesis: level of protein p53 and its activation by post-translational modifications/phosphorylation (SDS-PAGE, Western blot)

Main outcome of study (according to author)
The data indicated no significant changes in the expression and activation of the p53 protein between exposed and sham-exposed cells. The authors suggest that a radiofrequency field exposure at 900 MHz does not induce the p53 pathway and cellular stress.

(Study character: medical/biological study, experimental study, full/main study)

Study funded by

• Fondation Santé et Radiofréquences, France

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