The effects of exposure of human lung epithelial cells to a 60 Hz magnetic field alone or with co-exposure to gamma radiation or hydrogen peroxide on the cell cycle distribution and on aneuploidy should be investigated.
Cells were divided into the following groups: 1) exposure to a 1 mT magnetic field, 2) exposure to a 2 mT magnetic field, 3) exposure to hydrogen peroxide from the beginning of the exposure phase (0.05, 0.1, 0.5 mM), 4) exposure to gamma radiation at the beginning of the exposure phase (0.1, 0.5, 1, 2 Gy), 5) co-exposure to 2 mT magnetic field and 0.05 mM hydrogen peroxide simultaneously and 6) co-exposure to 2 mT magnetic field and 0.5 Gy gamma radiation (prior to magnetic field exposure). For each group and test, a separate sham exposure was conducted.
Each test was performed in triplicate and at least in four independent experiments.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
60 Hz
Exposure duration:
continuous for 9 hours
|
|
|
Exposure 2:
60 Hz
Exposure duration:
continuous for 9 hours
|
|
gamma radiation was applied as a single dose at the beginning of each exposure time, respectively, at a rate of 5 Gy/min
| Frequency | 60 Hz |
|---|---|
| Type | |
| Exposure duration | continuous for 9 hours |
| Exposure source |
|
|---|---|
| Chamber | 60 mm cell culture dishes |
| Setup | temperature was maintained at 37 ± 0.3°C |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 1 mT | - | - | - | - |
| Frequency | 60 Hz |
|---|---|
| Type | |
| Exposure duration | continuous for 9 hours |
| Exposure source |
|
|---|---|
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 2 mT | - | - | - | - |
No significant changes could be found between magnetic field exposed (groups 1 and 2) and sham exposed cells.
In groups 3 (hydrogen peroxide) and 4 (gamma radiation), significant changes in the cell cycle distribution and aneuploidy could be detected between exposed and sham exposed cells.
Exposure to the magnetic field and co-exposure to hydrogen peroxide (group 5) or gamma radiation (group 6) did not result in an enhancement of the effects of hydrogen peroxide or gamma radiation alone.
The authors conclude that exposure of human lung epithelial cells to a 60 Hz magnetic field has no effect on the cell cycle distribution and aneuploidy and does not enhance the effects of gamma radiation or hydrogen peroxide.
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