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Medical/biological Study (experimental study)

Electromagnetic fields at 2.45 GHz trigger changes in heat shock proteins 90 and 70 without altering apoptotic activity in rat thyroid gland. med./biol.

By: Misa Agustino MJ, Leiro JM, Jorge Mora MT, Rodriguez-Gonzalez JA, Jorge Barreiro FJ, Ares-Pena FJ, Lopez-Martin E
Published in: Biol Open 2012; 1 (9): 831 - 838 (full-text | PubMed | Journal website)

Aim of study (according to author)
To examine whether exposure to a 2.45 GHz electromagnetic field modifies the level of the heat shock proteins Hsp70 and Hsp90 and damages the morphological structure in the thyroid gland as well as to investigate whether apoptosis occurs.
Background/further details:
Female rats were distributed equally into two groups: 1.) sacrificed 90 min after exposure with different specific absorption rates: 1 a) 0 W, 1 b) 1.5 W, 1 c) 3 W and 1 d) 12 W (n= 6 per subgroup). 2.) sacrificed 24 h after exposure with different absorption rates: 2 a) 0 W, 2 b) 1.5 W, 2 c) 3 W and 2 d) 12 W (n= 6 per subgroup).

Endpoints

Exposure
General category: microwaves, 2.45 GHz

Field characteristicsParameters
field 1: 2.45 GHz
exposure duration: continuous for 30 min
power: 1.5 W
electric field strength: 28.48 V/m
SAR: 0.046 W/kg mean value (partial body) (in the thyroid)
SAR: 0.041 W/kg peak value (1 g) (in the thyroid)
SAR: 0.0169 W/kg mean value (whole body)
SAR: 0.089 W/kg peak value (1 g) (in the body)
field 2: 2.45 GHz
exposure duration: continuous for 30 min
power: 3 W
electric field strength: 40.28 V/m
SAR: 0.104 W/kg mean value (partial body) (in the thyroid)
SAR: 0.076 W/kg peak value (1 g) (in the thyroid)
SAR: 0.0364 W/kg mean value (whole body)
SAR: 0.18 W/kg peak value (1 g) (in the body)
field 3: 2.45 GHz
exposure duration: continuous for 30 min
power: 12 W
electric field strength: 80.56 V/m
SAR: 0.482 W/kg mean value (partial body) (in the thyroid)
SAR: 0.34 W/kg peak value (1 g) (in the thyroid)
SAR: 0.161 W/kg mean value (whole body)
SAR: 0.795 W/kg peak value (1 g) (in the body)

FIELD View further expo parameters

Exposed system:
animal (species/strain): rat/Sprague-Dawley
whole body exposure

Methods
Endpoint/Measurement parameters/Methodology

investigated material: DNA/RNA (in vitro), tissue slices (in vitro), tissue homogenates (thyroid gland)
investigation on living organism
investigated organ system: endocrine system, thyroid gland

time of investigation: before and after exposure

Main outcome of study (according to author)
90 minutes after the exposure to 1.5 W, a significant decrease in Hsp70 levels occurred in comparison to the control. Hsp90 levels were significantly decreased 90 minutes after the exposure at power levels of 1.5 W and 3 W, as well as 24 hours after the exposure at a power level of 3 W compared to the control. Tissue slices of the thyroid gland of exposed animals (1.5 W and 3 W) did not show any morphological alterations when compared to unexposed animals. 90 minutes and 24 hours after the exposure at 12 W, only a slight loss of cell adhesion was observed in some thyroid gland follicles. No indication for apoptosis was observed in tissue slices from exposed (all groups and different specific absorption rates) or sham exposed animals.
The results suggest that exposure to a 2.45 GHz electromagnetic field may alter levels of cellular stress in rat thyroid gland.

(Study character: medical/biological study, experimental study, full/main study)

Study funded by

Related articles i
Glossary: absorption, animals, apoptosis, biological, biosynthesis, cell adhesion, cell division, cellular, cell viability, DAPI, DNA, electric field strength, electromagnetic field, ELISA, endocrine system, endpoint, exposed, exposure, fluorescence microscopy, follicles, full/main study, GHz, heat shock proteins, hematoxylin-eosin stain, histopathological, Homogenates, Hsp70, Hsp90, in vitro, lesions, light microscopy, mean value, microwaves, molecular, morphological, organism, power, proliferation, rat, rat/Sprague-Dawley, rectal temperature, RNA, sham exposed, significant, species, specific absorption rates, strain, stress, thyroid gland, tissue, whole body exposure
Exposure: microwaves, 2.45 GHz

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