Medical/biological Study (experimental study)

Effect of acute extremely low frequency electromagnetic field exposure on the antioxidant status and lipid levels in rat brain. med./biol.

PubMed Entry

Journal web site

Aim of study (according to author)
To study the immediate effects of acute magnetic field exposure, immobilization, and the combination of both on the antioxidant system and lipid content in the whole brain of rat.
Background/further details:
32 male rats were divided into four groups: 1) control group, 2) exposure group, 3) immobilization, 4) exposure + immobilization.
Immobilization was used as a positive control for physical and psychological stress.

Endpoint

• effects on the neurological system: oxidative stress in the brain, brain metabolism

Exposure
General category: magnetic field, low frequency field, 50/60 Hz (AC)

Field characteristics	Parameters
60 Hz exposure duration: continuous for 2 h	magnetic flux density: 2.4 mT

Exposed system:
animal (species/strain): rat/Wistar
whole body exposure

Methods
Endpoint/Measurement parameters/Methodology

• endocrine changes: plasma corticosterone content
• effects on the neurological system: oxidative stress in the brain and brain metabolism (level of reduced glutathione), enzyme activities of catalase and superoxide dismutase (spectrophotometry); level of nitric oxide (colorimetry); total lipid content and thiobarbituric acid reactive substances in total lipid content; triacylglycerol and cholesterol levels (commercial kits)

Main outcome of study (according to author)
Acute exposure to extremely low frequency magnetic field alone as well as "exposure + immobilization" induced a reduction in catalase and superoxide dismutase enzyme activities compared with the control group, whereas the combination of "magnetic field exposure and immobilization" also decreased both reduced glutathione (compared with the control group) and nitric oxide levels compared with the other groups. Thiobarbituric acid reactive substances in the total lipid content of the brain were higher in the "exposure + immobilization group" than in the other experimental groups. Exposure to extremely low frequency magnetic fields did not induce any changes of the stress-hormone corticosterone or triacylglycerol levels.
Acute exposure to the extremely low frequency magnetic field appears to be a mild stressor.

(Study character: medical/biological study, experimental study, full/main study)

Study funded by

• Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica (PAPIIT), Universidad Nacional Autonoma de Mexico (UNAM), Mexico
• Universidad Nacional Autònoma de México (UNAM)

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