Medical/biological Study (experimental study)
Determination of death thresholds and identification of Terahertz (THz)-specific gene expression signatures. med./biol. By: Wilmink GJ, Ibey BL, Roth CL, Vincelette RL, Rivest BD, Horn CB, Bernhard J, Roberson D, Roach WP
Published in: Jansen ED, Thomas RJ (2010): "Optical interactions with tissues and cells XXI: 25 - 27 January 2010, San Francisco, California, United States", SPIE (ISBN 97880819479587, 280 pages): 7562 OK-1 - 7562 OK-8
Aim of study (according to author)
To study if high-power THz radiation causes cellular and/or molecular effects in human Jurkat cells and dermal fibroblast cell lines.
General category: terahertz fields
FIELD View further expo parameters
intact cell/cell culture (in vitro)
Jurkat cells (human lymphoblastoid T cells) and human dermal fibroblasts
Methodsinvestigated material: DNA/RNA (in vitro), intact cell/cell culture (in vitro)
time of investigation: after exposure
Main outcome of study (according to author)
The MTT assay showed that 60% of the Jurkat cells survived the 30-minutes THz exposure, whereas only 20% survived the 40-minutes exposure. The flow cytometry data showed similar results and provided additional evidence suggesting that THz-induced cell death was mediated by both necrotic and apoptotic processes.
The preliminary confocal microscopy data suggested that cells exposed to THz radiation for 12 seconds or longer show appreciable signs of cell death.
The preliminary microarray data showed that dermal fibroblasts upregulated several genes when exposed to THz irradiation (primarily inflammatory cytokines).
Overall, these findings provide evidence for the cellular and molecular effects of THz irradiation.
(Study character: medical/biological study, experimental study, full/main study, pilot/exploratory/preliminary study)
Study funded by
- Air Force Research Laboratory's (AFRL) Human Effectiveness Directorate, USA
- HQ AF/SGRS (Headquarters United States Air Force/Surgeon General Research and Development Divison), USA
- Alexandrov BS et al. (2011): Non-thermal effects of terahertz radiation on gene expression in mouse stem...
- Hintzsche H et al. (2011): Terahertz radiation induces spindle disturbances in human-hamster hybrid cells.
- Wilmink GJ et al. (2011): In vitro investigation of the biological effects associated with human dermal...
- Bock J et al. (2010): Mammalian stem cells reprogramming in response to terahertz radiation.
- Alexandrov BS et al. (2010): DNA Breathing Dynamics in the Presence of a Terahertz Field.
- Korenstein-Ilan A et al. (2008): Terahertz radiation increases genomic instability in human lymphocytes.
- Bourne N et al. (2008): The effects of terahertz radiation on human keratinocyte primary cultures and...
- Zeni O et al. (2007): Cytogenetic Observations In Human Peripheral Blood Leukocytes Following In...
- Clothier RH et al. (2003): Effects of THz exposure on human primary keratinocyte differentiation and...
- Scarfi MR et al. (2003): THz Exposure of Whole Blood for the Study of Biological Effects on Human...
- Smye SW et al. (2001): The interaction between Terahertz radiation and biological tissue.
Glossary: adherent, annexin V, apoptotic, biological, biosynthesis, cell culture, cell death, cell division, cell lines, cells, cellular, cell viability, confocal microscopy, cytokines, dermal, DNA, endpoint, evidence, exposed, exposure, fibroblasts, FITC, flow cytometry, full/main study, gene expression, genes, human, induced, inflammatory, in vitro, irradiation, Jurkat cells, lymphoblastoid, microarray, molecular, mRNA, MTT assay, necrotic, pilot/exploratory/preliminary study, power, power flux density, proliferation, propidium iodide, radiation, RNA, T cells, THz, upregulated
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