Medical/biological Study (experimental study)

Cell viability and growth in a battery of human breast cancer cell lines exposed to 60 Hz magnetic fields. med./biol.

PubMed Entry

Journal web site

Aim of study (according to author)
The cell viability and cell growth of normal breast cells and breast cancer cells exposed to low frequency magnetic field in vitro should be investigated.
Background/further details:
Four human breast cancer cell lines were selected differing in their hormone receptor status to clarify the hypothesis whether hormone-dependent cells react more sensitive to magnetic field.
In a further step the role of magnetic field exposure on artificial induced cytotoxicity was examined.

Endpoint

• cell viability/cell division/proliferation: cell viability, cell growth

Exposure
General category: magnetic field, 50/60 Hz (AC)

Field characteristics	Parameters
60 Hz exposure duration: continuous for 72 h	magnetic flux density: 1 mT

Exposed system:
intact cell/cell culture (in vitro)
MCF-7, ZR-75-1, T-47D, and MDA-MB-231 (breast cancer cell line) and normal human breast epithalial cells

Methods
Endpoint/Measurement parameters/Methodology

• cell viability/cell division/proliferation: cell viability (sulforhodamine B assay, sulforhodamine B binds total cellular protein, can be measured by colorimetry and reflects the cell number), cell growth
• others: cytotoxicity (treatment with apoptosis inducing retinoid, sulforhodamine B assay)

Main outcome of study (according to author)
Magnetic field exposure had no effect on cell viability or cell growth in any cell line examined. Similarly, magnetic field exposure had no effect on artificial induced cytotoxicity.
The results show that magnetic field exposure does not exert influence on cell growth in breast cancer cells in vitro.

(Study character: medical/biological study, experimental study, full/main study)

Study funded by

• National Institute of Environmental Health Sciences (NIEHS), North Carolina, USA

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