Medical/biological Study (experimental study)
Modulation of heat shock protein response in SH-SY5Y by mobile phone microwaves. med./biol. By: Calabro E, Condello S, Curro M, Ferlazzo N, Caccamo D, Magazu S, Ientile R
Published in: World J Biol Chem 2012; 3 (2): 34 - 40 ( full article, PubMed Entry , Journal web site )
Aim of study (according to author)
To study the effects of radiofrequency exposure on the stress response in neuroblastoma cells, differentiated to dopaminergic neuron-like cells (induced by retinoic acid).
General category: mobile communication system, mobile phone, GSM
FIELD View further expo parameters
intact cell/cell culture (in vitro)
SH-SY5Y (human neuroblastoma cell line)
Methodsinvestigated material: isolated bio./chem. substance (in vitro), intact cell/cell culture (in vitro), cell lysates
time of investigation: after exposure
Main outcome of study (according to author)
Under these experimental conditions, neither apoptosis nor Hsp27 expression nor caspase 3 expression were significantly changed following exposure. However, a significant decrease in Hsp20 expression was found at both times of exposure (2 and 4 h), whereas Hsp70 levels were significantly increased only after 4 h exposure (according to the abstract; within the results section, the authors also demonstrate a significant increase after the 2 h exposure compared with the sham exposed cells, remark EMF-Portal). Additionally, p-Hsp27 (phosphorylated Hsp27) expression was significantly increased in the exposed cells compared with the controls.
Remark EMF-Portal: The data on the cell viability are not clearly documented according to significance (in the abstract and discussion section: no change; within the results section: decrease), c.f. full text link.
The changes in the expression of the heat shock proteins in neuronal cells could be an early response to the microwave exposure.
(Study character: medical/biological study, experimental study, full/main study)
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Glossary: acid, apoptosis, biological, biosynthesis, caspase, cell culture, cell division, cell line, cells, cellular, cell viability, differentiated, dopaminergic, electric field strength, EMF, endpoint, exposed, exposure, expression, full/main study, GSM, heat shock proteins, Hsp27, Hsp70, Hsp90, human, induced, in vitro, lysates, magnetic field strength, microwave, mobile communication, mobile phone, molecular, MTT assay, neuroblastoma, neuronal, phosphorylated, power flux density, proliferation, radiofrequency, SAR, sham exposed, significance, significant, stress, Western blot
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