Medical/biological Study (experimental study)

Effect of exposure to extremely low electro-magnetic field during prenatal period on mice spleen. med./biol.

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Aim of study (according to author)
To study the effects of extremely low frequency magnetic fields on the embryogenesis of mice, particularly on the development of the spleen.
Background/further details:
48 pregnant mice were divided into six groups (each group n=8): 1) control group, 2) sham exposed group, 3) - 6) exposure groups starting with exposure on different days of gestation (1-5, 6-10, 11-15, 16-20).

Endpoint

• effects on embryo/fetus: embryogenesis (spleen development)

Exposure
General category: magnetic field, low frequency field, 50/60 Hz (AC)

Field characteristics	Parameters
50 Hz exposure duration: continuous for 10 h/day on 5 days	magnetic flux density: 6 mT

Exposed system:
animal (species/strain): mouse/NMRI
whole body exposure

Methods
Endpoint/Measurement parameters/Methodology

• effects on embryo/fetus: embryogenesis (spleen volume, number of spleen megakaryocytes (stereological analysis; hematoxylin-eosin stain), body weight of newborns (n=180))

Main outcome of study (according to author)
Total body weight of newborns, the volume of spleen, and the number of megakaryocytes decreased following the exposure to extremely low frequency electromagnetic fields. The complete period of gestation was sensitive to exposure and the initial days were more sensitive, i.e. the effects were more pronounced.

(Study character: medical/biological study, experimental study, full/main study)

Study funded by

• Arak University, Iran

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