Medical/biological Study (experimental study)
Clinical significance of different effects of static and pulsed electromagnetic fields on human osteoclast cultures. med./biol. By: Barnaba SA, Ruzzini L, Di Martino A, Lanotte A, Sgambato A, Denaro V
Published in: Rheumatol Int 2012; 32 (4): 1025 - 1031 ( PubMed Entry , Journal web site )
Aim of study (according to author)
To study whether static magnetic fields that are generated around spinal metal (titanium) devices could influence periprosthetic osteolysis modulating not only osteoblasts but also osteoclast cells function. Additionally, it was investigated whether pulsed electromagnetic fields that are usually used in clinical practice for nonunions could affect osteoclasts differentiation and activity.
Primary osteoclast cells were isolated from primary human osteoclast precursor cells.
General category: static magnetic field, low frequency field, signals/pulses, 50/60 Hz (AC), DC
|field 1: DC/static|
exposure duration: continuous for 7, 10 or 14 days
|magnetic flux density: 0.9 µT |
|field 2: 50 Hz |
exposure duration: continuous for 7, 10 or 14 days
|magnetic flux density: 0.4 mT |
FIELD View further expo parameters
intact cell/cell culture (in vitro)
Methodsinvestigated material: intact cell/cell culture (in vitro), cell lysates
time of investigation: after exposure
Main outcome of study (according to author)
The static magnetic field-exposed cells showed a more differentiated phenotype, a higher number of differentiated cells and a significantly higher TRAP enzyme activity after 7 and 10 days of exposure with respect to a sham exposed cells. Pulsed electromagnetic field exposed cells have a less-differentiated phenotype after 7 days of exposure compared with the relative sham exposed control (no difference after 10 and 14 days), while the TRAP enzyme activity showed no statistically significant differences between exposed and control cells at any observation time.
The findings indicated that static magnetic fields of the same intensity of the one generated around spinal devices can affect osteoclast cell differentiation and activity. Aseptic loosening around titanium implants might be due in part to an increased osteoclast activity and cell differentiation. Pulsed electromagnetic fields of the same intensity than the one used for the management of nonunions can affect osteoclasts phenotype after 7 days of exposure, while osteoclasts TRAP enzyme activity is not affected by this kind of electromagnetic fields.
(Study character: medical/biological study, experimental study, pilot/exploratory/preliminary study)
Study funded byRelated articles
- Jing D et al. (2011): The preventive effects of pulsed electromagnetic fields on diabetic bone loss...
- Denaro V et al. (2011): Effect of pulsed electromagnetic fields on human tenocyte cultures from...
- Akdag MZ et al. (2010): The effect of long-term extremely low-frequency magnetic field on geometric and...
- Lin HY et al. (2010): Repairing large bone fractures with low frequency electromagnetic fields.
- Shen WW et al. (2010): Pulsed electromagnetic fields stimulation affects BMD and local factor...
- Li KC et al. (2009): Effects of electromagnetic pulse on bone metabolism of mice in vivo.
- Akpolat V et al. (2009): Treatment of osteoporosis by long-term magnetic field with extremely low...
- Zhao D et al. (2008): Electromagnetic field change the expression of osteogenesis genes in murine...
- Denaro V et al. (2008): Static electromagnetic fields generated by corrosion currents inhibit human...
- Marquez-Gamino S et al. (2008): Pulsed electromagnetic fields induced femoral metaphyseal bone thickness...
- Gurgul S et al. (2008): Deterioration of bone quality by long-term magnetic field with extremely low...
- Zhang XY et al. (2006): Effects of 0.4 T rotating magnetic field exposure on density, strength, calcium...
- Sul AR et al. (2006): Effects of sinusoidal electromagnetic field on structure and function of...
- Chang K et al. (2006): Pulsed electromagnetic fields accelerate apoptotic rate in osteoclasts.
- Okudan B et al. (2006): DEXA analysis on the bones of rats exposed in utero and neonatally to static...
- Hannay G et al. (2005): Timing of pulsed electromagnetic field stimulation does not affect the...
- Chang K et al. (2005): Pulsed electromagnetic fields stimulation affects osteoclast formation by...
- Wang W et al. (2004): Up-regulation of chondrocyte matrix genes and products by electric fields.
- Chang K et al. (2003): Effects of different intensities of extremely low frequency pulsed...
- Diniz P et al. (2002): Effects of pulsed electromagnetic field (PEMF) stimulation on bone tissue like...
- Guerkov HH et al. (2001): Pulsed electromagnetic fields increase growth factor release by nonunion cells.
- Lohmann CH et al. (2000): Pulsed electromagnetic field stimulation of MG63 osteoblast-like cells affects...
- Lee JH et al. (2000): Morphologic responses of osteoblast-like cells in monolayer culture to ELF...
- Grace KL et al. (1998): The effects of pulsed electromagnetism on fresh fracture healing: osteochondral...
- Heermeier K et al. (1998): Effects of extremely low frequency electromagnetic field (EMF) on collagen type...
- Tabrah FL et al. (1998): Clinical report on long-term bone density after short-term EMF application.
- Simske SJ et al. (1995): Suspension osteopenia in mice: whole body electromagnetic field effects.
- Farndale RW et al. (1985): Pulsed electromagnetic fields promote collagen production in bone marrow...
- Bassett CA et al. (1974): Acceleration of fracture repair by electromagnetic fields. A surgically...
Glossary: 50/60 Hz, AC, acid, biological, bone, cell culture, cell morphology, cells, DC, differentiated, electromagnetic fields, endpoint, enzyme activity, exposed, exposure, expressed, human, implants, in vitro, low frequency field, lysates, magnetic flux density, Nuclei, osteoblasts, osteoclast, phase contrast microscopy, phenotype, phosphatase, pilot/exploratory/preliminary study, precursor cells, pulsed electromagnetic field, pulses, resorption, sham exposed, signals, significant, spectrophotometry, static magnetic field, statistically
© 1997 - 2013, Research Center for Bioelectromagnetic Interaction (femu - RWTH Aachen University, Germany).
The informational contents of the EMF-Portal are available free of charge for personal and strictly non-commercial purposes. The informational contents of the EMF-Portal may be retrieved, read or printed, but not (i) copied, (ii) changed or (iii) saved in any format, neither electronically nor on other storage media. Permissions for publication, reproduction, commercial purposes or third party propagation of contents of the EMF-Portal – including partial excerpts or revised formats – have to be obtained from the femu Aachen University-copyright holders. By retrieving, reading or printing these documents you expressly state your agreement with all conditions in the fine print.