Medical/biological Study (experimental study)

Effects of extremely low-frequency magnetic field on caspase activities and oxidative stress values in rat brain. med./biol.

PubMed Entry

Journal web site

Aim of study (according to author)
To study the effects of 100 µT and 500 µT extremely low frequency magnetic fields, which are the limit values for public environmental and occupational magnetic field exposure, on apoptosis and oxidative stress values in the brains of rats (each group n=10).

Endpoint

• effects on the neurological system: apoptosis and oxidative stress in the brain

Exposure
General category: magnetic field, low frequency field, 50/60 Hz (AC)

Field characteristics	Parameters
50 Hz exposure duration: continuous for 2 hr/day, 7 days/week, for 10 months	magnetic flux density: 100 µT (limit for public exposure) magnetic flux density: 500 µT (limit for occupational exposure)

Exposed system:
animal (species/strain): rat/Sprague-Dawley
whole body exposure

Methods
Endpoint/Measurement parameters/Methodology

• effects on the neurological system: apoptosis (active (cleaved) caspase-3) and oxidative stress (levels of malondialdehyde (for lipid peroxidation evaluation), myeloperoxidase and catalase enzyme activities, total antioxidant capacity (TAC), total oxidant status (TOS), oxidative stress index (TOS/TAC); spectrophotometry)

Main outcome of study (according to author)
Apoptosis and myeloperoxidase enzyme activity were not significantly different between the groups. Catalase enzyme activity decreased in both exposure groups, while total antioxidant capacity was lower in the 500 µT exposed group than those in the 100 µT and the sham exposure groups. Malondialdehyde, total oxidant status, and oxidative stress index values were found to be higher in the 500 µT group than those in the 100 µT and the sham exposure groups.
The authors conclude, that apoptosis was not changed by long-term extremely low frequency magnetic field exposure, while both 100 µT and 500 µT extremely low frequency magnetic field exposure induced a toxic effect in the rat brain by increasing oxidative stress and diminishing antioxidant defense system.

(Study character: medical/biological study, experimental study, full/main study, blind study)

Study funded by

• not stated/none

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