Medizinisch/biologische Studie (experimentelle Studie)

An in vitro study of the effects of exposure to a GSM signal in two human cell lines: monocytic U937 and neuroblastoma SK-N-SH. med./biol.

PubMed Eintrag

Journal Website

Ziel der Studie (lt. Autor)
Diese in vitro-Studie wurde durchgeführt, um die möglichen biologischen Wirkungen eines GSM-ähnlichen Signals auf die Zelllebensfähigkeit und Genexpression (z.B. Hitzeschock-Protein) bei zwei menschlichen Zelllinien zu untersuchen.

Endpunkte

• molekulare Biosynthese: Genexpression
• Zelllebensfähigkeit/Zellteilung/Zellproliferation: Zelllebensfähigkeit

Exposition/Befeldung
Allgemeine Kategorie: Mobilkommunikationssystem, digitales Mobiltelefon, GSM

Feldeigenschaften	Parameter
900 MHz gepulst (PW) Expositionsdauer: kontinuierlich für 1 oder 2 h	SAR: 0,2 W/kg

Exponiertes System:
intakte Zelle/Zellkultur (in vitro)
menschliche Neuroblastom-Zelllinie (SK-N-SH) und Monozyten-Zelllinie (U937)

Methoden
Endpunkt/Messparameter/Methodik

• molekulare Biosynthese: Gehalt an Hitzeschock-Protein (Hsp60, Hsp70, Hsp90, Western Blot), Gen-Transkription (LIM, Nap1L1, CCPG1, ACADM, BMAL1, Rbbp4, RT-PCR)
• Zelllebensfähigkeit/Zellteilung/Zellproliferation: Zelllebensfähigkeit (Trypanblau-Ausschluss-Assay), Nekrose/Apoptose, Zellzyklus-Verteilung (Propidiumiodid-Färbung, Durchflusszytometrie)

Hauptergebnis der Studie (lt. Autor)
Zwischen den schein-exponierten und Hochfrequenz-exponierten Zellen wurden keine signifikanten Unterschiede in der Zelllebensfähigkeit, in der Zellzyklus-Verteilung oder der Genexpression festgestellt.

(Studienmerkmale: medizinisch/biologische Studie, experimentelle Studie, Voll-/Hauptstudie)

Studie gefördert durch

• Key University Research Centre for Health Technologies, University of Technology Sydney, Australia

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