Medical/biological Study (experimental study)

Effects of global system for mobile communications 1800 MHz radiofrequency electromagnetic fields on gene and protein expression in MCF-7 cells. med./biol.

PubMed Entry

Journal web site

Aim of study (according to author)
To study the effects of radiofrequency electromagnetic field exposure on gene and protein expression profiles in human breast cancer cell line MCF-7.

Endpoint

• molecular biosynthesis: protein and gene expression

Exposure
General category: mobile communication system, digital mobile phone, GSM

Field characteristics	Parameters
field 1: 1800 MHz pulsed (PW) exposure duration: intermittent, 5 min on/10 min off, for 24 h transcriptome analysis	SAR: 2 W/kg mean value SAR: 3.5 W/kg mean value
field 2: 1800 MHz pulsed (PW) exposure duration: intermittent, 5 min on/10 min off, for 1, 3, 6, 12, and 24 h proteome analysis	SAR: 3.5 W/kg mean value
field 3: 1800 MHz pulsed (PW) exposure duration: continuous for 1, 3, 6, 12, and 24 h proteome analysis	SAR: 3.5 W/kg mean value

Exposed system:
intact cell/cell culture (in vitro)
MCF-7 (human breast cancer cell line)

Methods
Endpoint/Measurement parameters/Methodology

• molecular biosynthesis: protein and gene expression (Affymetrix GeneChip HG-U133A (DNA microarray); RT-PCR; two-dimensional gel electrophoresis)

Main outcome of study (according to author)
DNA microarray analysis identified a handful of consistent changed genes after exposure to radiofrequency electromagnetic field at specific absorption rates of up to 3.5 W/kg for 24 h. However, these differentially transcribed genes could not be further confirmed by RT-PCR assay.
Meanwhile, systematic proteome analysis revealed that a few but different proteins were differentially expressed under continuous or intermittent radiofrequency electromagnetic field exposure at SAR of 3.5 W/kg for 24 h or less, implying that the observed effects might have occurred by chance.
In conclusion, the data do not provide convincing evidence that radiofrequency electromagnetic field exposure under the present experimental conditions can produce distinct effects on gene and protein expression in the MCF-7 cells.

(Study character: medical/biological study, experimental study, full/main study)

Study funded by

• Ministry of Education, China
• National Natural Science Foundation (NSFC), China
• Zhejiang Provincial Health Bureau, China
• Zhejiang Provincial Key Projects for Science and Technology, China
• Zhejiang Provincial Natural Science Foundation, China

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