Medical/biological Study (experimental study)
Effects of global system for mobile communications 1800 MHz radiofrequency electromagnetic fields on gene and protein expression in MCF-7 cells. med./biol. By: Zeng Q, Chen G, Weng Y, Wang L, Chiang H, Lu D, Xu Z
Published in: Proteomics 2006; 6 (17): 4732 - 4738 ( PubMed Entry , Journal web site )
Aim of study (according to author)
To study the effects of radiofrequency electromagnetic field exposure on gene and protein expression profiles in human breast cancer cell line MCF-7.
General category: mobile communication system, digital mobile phone, GSM
|field 1: 1800 MHz |
exposure duration: intermittent, 5 min on/10 min off, for 24 h
|SAR: 2 W/kg mean value|
SAR: 3.5 W/kg mean value
|field 2: 1800 MHz |
exposure duration: intermittent, 5 min on/10 min off, for 1, 3, 6, 12, and 24 h
|SAR: 3.5 W/kg mean value|
|field 3: 1800 MHz |
exposure duration: continuous for 1, 3, 6, 12, and 24 h
|SAR: 3.5 W/kg mean value|
FIELD View further expo parameters
intact cell/cell culture (in vitro)
MCF-7 (human breast cancer cell line)
Methodsinvestigated material: isolated bio./chem. substance (in vitro), DNA/RNA (in vitro)
time of investigation: after exposure
Main outcome of study (according to author)
DNA microarray analysis identified a handful of consistent changed genes after exposure to radiofrequency electromagnetic field at specific absorption rates of up to 3.5 W/kg for 24 h. However, these differentially transcribed genes could not be further confirmed by RT-PCR assay.
Meanwhile, systematic proteome analysis revealed that a few but different proteins were differentially expressed under continuous or intermittent radiofrequency electromagnetic field exposure at SAR of 3.5 W/kg for 24 h or less, implying that the observed effects might have occurred by chance.
In conclusion, the data do not provide convincing evidence that radiofrequency electromagnetic field exposure under the present experimental conditions can produce distinct effects on gene and protein expression in the MCF-7 cells.
(Study character: medical/biological study, experimental study, full/main study)
Study funded by
- Ministry of Education, China
- National Natural Science Foundation (NSFC), China
- Zhejiang Provincial Health Bureau, China
- Zhejiang Provincial Key Projects for Science and Technology, China
- Zhejiang Provincial Natural Science Foundation, China
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Glossary: assay, biological, biosynthesis, breast cancer, cell culture, cell line, cells, digital, DNA, DNA microarray, electromagnetic field, endpoint, evidence, exposure, expressed, full/main study, gene, gene expression, GSM, human, intermittent, in vitro, mean value, mobile communication, mobile phone, molecular, protein expression, Proteins, proteome, pulsed, PW, radiofrequency, RNA, RT-PCR, SAR, transcribed, transcriptome, two-dimensional gel electrophoresis
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