Medical/biological Study (experimental study)
2.45 GHz radiofrequency fields alter gene expression in cultured human cells. med./biol. By: Lee S, Johnson D, Dunbar K, Dong H, Ge X, Kim YC, Wing C, Jayathilaka N, Emmanuel N, Zhou CQ, Gerber HL, Tseng CC, Wang SM
Published in: FEBS Lett 2005; 579 (21): 4829 - 4836 ( PubMed Entry , Journal web site )
Aim of study (according to author)
To study whether radiofrequency fields can cause changes in gene expression in cultured human cells. The authors aimed to provide genome-wide coverage of the expressed genes regardless their functional categories in the radiofrequency treated cells to address if radiofrequency has biological effects.
General category: 2.45 GHz
FIELD View further expo parameters
intact cell/cell culture (in vitro)
HL-60 (human acute myeloid leukaemia cells)
Methodsinvestigated material: DNA/RNA (in vitro)
time of investigation: after exposure
Main outcome of study (according to author)
The authors revealed that 221 genes altered their expression after a 2 h exposure. The number of affected genes increased to 759 after a 6 h exposure. Functional classification of the affected genes showed that apoptosis-related genes were among the upregulated ones and the cell cycle genes among the downregulated ones. No significant increase in the expression of heat shock protein genes was found.
These data suggest that the radiofrequency fields at 2.45 GHz can alter gene expression in cultured human cells through non-thermal mechanism.
(Study character: medical/biological study, experimental study, full/main study)
Study funded by
- Air Force Office of Scientific Research/Department of Defense Multidisciplinary University Research Initiative (AFOSR/DOD MURI), USA
- Department of Defense (DoD), USA
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Glossary: acute myeloid leukaemia, apoptosis, biological, biosynthesis, cell cycle, cells, cultured, DNA, downregulated, electric field strength, endpoint, exposure, expressed, expression, full/main study, gene expression, genes, genome, GHz, heat shock protein, HL-60, human, in vitro, mean value, molecular, non-thermal, pulsed, PW, radiofrequency, RNA, SAR, serial analysis of gene expression, significant, upregulated
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