The effects of exposure of mouse embryonic fibroblasts to a 1800 MHz electromagnetic field on oxidative stress, DNA damage and apoptosis should be investigated.
Cells were divided into the following groups: exposure to the electromagnetic field for 1) 0.5 h, 2) 1 h, 3) 1.5 h, 4) 2 h, 5) 4 h, 6) 6 h or 7) 8 hours. For each group, a corresponding sham exposure group was created.
Positive controls were conducted and each experiment was repeated thrice.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
1,800 MHz
Modulation type:
pulsed
|
| Frequency | 1,800 MHz |
|---|---|
| Type | |
| Exposure duration | intermittent exposure (5 min on/10 min off) for 0.5, 1, 1.5, 2, 4, 6 or 8 hours |
| Exposure source | |
|---|---|
| Chamber | culture dishes |
| Setup | two waveguides, one for exposure and the other one for sham exposure, were placed in incubator; culture dishes in dish holder were placed inside the waveguide in the H-field maximum of the standing wave and E-polarization; the temperature was kept at 37 ± 0.1°C |
| Sham exposure | A sham exposure was conducted. |
| Additional info | temperature differences between the exposed and the sham exposed cultures never exceeded 0.1°C |
There were no significant differences in the amount of DNA damages between the exposure groups and their corresponding sham exposure groups.
The intracellular level of reactive oxygen species was significantly increased after 1, 4 and 8 hours of exposure compared to the corresponding sham exposure groups.
After exposure for 1, 4 or 8 h, the number of late-apoptotic cells increased significantly compared to the respective sham-exposed groups as well.
The authors conclude that exposure of mouse embryonic fibroblasts to a 1800 MHz electromagnetic field could promote oxidative stress and apoptosis.
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