Study type: Medical/biological study (experimental study)

Effect of 60 Hz electromagnetic fields on the activity of hsp70 promoter: an in vivo study med./bio.

By: Rodriguez-De la Fuente AO, Alcocer-Gonzalez JM, Heredia-Rojas JA, Rodriguez-Padilla C, Rodriguez-Flores LE, Santoyo-Stephano MA, Castaneda-Garza E, Tamez-Guerra RS
Published in: Cell Biol Int Rep 2012; 19 (1): e00014
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Aim of study (acc. to author)

To investigate the effects of exposure to an extremely low frequency magnetic field on the expression of the luciferase gene in the quadriceps muscle from mice.

Background/further details

Additionally, the findings of Lin et al. should be confirmed, who claimed that the hsp70 promoter responses to magnetic fields.
A plasmid that includes the luciferase gene and partially the hsp70 promoter was electro-transfected in vivo into the quadriceps muscle (skeletal muscle of the femur) of mice.
Four groups were examined (n=3 per group): 1.) non-transfected mice (negative control), 2.) transfected mice (plasmid containing hsp70 promoter), 3.) transfected mice (plasmid without the hsp70 promoter) and 4.) transfected mice (plasmid containing hsp70 promoter) + magnetic field exposure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 2 h/day on 7 days

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration continuous for 2 h/day on 7 days
Exposure setup
Exposure source
Setup 71 cm long solenoid with a radius of 13.5 cm constructed by 552 turns of 1.3 mm diameter enamel-insulated copper wire; plastic separator placed inside the coil to allow the placement of uncaged mice in the coil; food and water containers also placed inside the coil
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 80 µT effective value measured - -
Additional parameter details

ambient AC field < 0.4 µT average geomagnetic field: 20 µT

Reference articles

  • Heredia-Rojas JA et al. (2004): Lack of alterations on meiotic chromosomes and morphological characteristics of male germ cells in mice exposed to a 60 Hz and 2.0 mT magnetic field

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In the quadriceps muscle of exposed mice transfected with the plasmid containing the hsp70 promoter (group 4), the enzyme activity of the luciferase was significantly increased (87.6% when compared to group 2) compared to all other groups.
The authors conclude that exposure to an extremely low frequency magnetic field increased the luciferase gene expression by activation of the hsp70 promoter in the quadriceps muscle of mice.

Study character:

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