Medical/biological study (experimental study)

Effect of alternating magnetic field treatments on enzymatic parameters of cellulase med./bio.

By: Zhang J, Wang S, Xu B, Gao M

Published in: J Sci Food Agric 2012; 92 (7): 1384-1388

Download citation in RIS format

Aim of study (acc. to author)

To evaluate the enzymatic parameters of cellulase exposed to an alternating magnetic field.

Endpoint

enzyme activity of cellulase

Exposure

- 50 Hz
- 50/60 Hz
- magnetic field
- low frequency

Exposure	Parameters
Exposure 1: 50 Hz Exposure duration: continuous for up to 60 min	magnetic flux density: 4.2 mT maximum

Exposure 1

Main characteristics

Frequency	50 Hz
Type	magnetic field
Exposure duration	continuous for up to 60 min

Exposure setup

Exposure source	coil(s)
Setup	six sets of three cylindrical coils with a radius of 125 mm positioned on a 1500 mm x 750 mm shaker board; glass bottles with the samples placed in the center of the coils
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	4.2 mT	maximum	-	-	-

Additional parameter details

samples were exposed to 18 different magnetic fields (magnetic flux densities not exactly specified, beside the maximum value of 4.2 mT)

Reference articles

Gao M et al. (2011): Extremely low frequency magnetic field effects on metabolite of Aspergillus niger

Exposed system:

isolated bio./chem. substance
enzyme solution (cellulase), cellulose

Methods Endpoint/measurement parameters/methodology

kinetic of the cellulase (enzyme activity, Linewaver-Burk plot, Michaelis-Menten constant) using carboxymethyl cellulose as a substrate (concentration of glucose, spectrophotometry)

Investigated system:

isolated bio./chem. substance
enyme solution

Time of investigation:

during exposure
after exposure

Main outcome of study (acc. to author)

The maximum and minimum enzyme activities of cellulase occurred at magnetic fields of 2.2 mT and 4.2 mT respectively after 20 minutes.
The authors conclude that treating the enzyme/substrate solutions with different magnetic fields could inhibit or facilitate the enzyme activity.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

Hubei Provincial Science and Technology Department, China
Yangtze University, China

Batcioglu K et al. (2011): Investigation of a weak magnetic field effect on the in vitro catalytic activity of adenosine deaminase and xanthine oxidase
Ravera S et al. (2010): Sinusoidal ELF magnetic fields affect acetylcholinesterase activity in cerebellum synaptosomal membranes
Prashanth KS et al. (2009): Effect of 50 Hz electromagnetic fields on acid phosphatase activity
Morre DJ et al. (2008): Response of the regulatory oscillatory behavior of copperII-containing ECTO-NOX proteins and of CuIICl2 in solution to electromagnetic fields
Prashanth KS et al. (2008): Effect of 50 Hz electromagnetic fields on Alpha amylase activity
Morelli A et al. (2005): Effects of extremely low frequency electromagnetic fields on membrane-associated enzymes
Portaccio M et al. (2005): Modulation of the catalytic activity of free and immobilized peroxidase by extremely low frequency electromagnetic fields: dependence on frequency
Markov MS (2004): Myosin Light Chain Modification Depending on Magnetic Fields: II. Experimental
Portaccio M et al. (2003): In vitro studies of the influence of ELF electromagnetic fields on the activity of soluble and insoluble peroxidase
Taoka S et al. (1997): Magnetic field effects on coenzyme B12-dependent enzymes: validation of ethanolamine ammonia lyase results and extension to human methylmalonyl CoA mutase
Harkins TT et al. (1994): Magnetic field effects on B12 ethanolamine ammonia lyase: evidence for a radical mechanism
Vajda T (1980): Investigation of magnetic field effect on trypsin activity