Combined effects of 872 MHz radiofrequency radiation and ferrous chloride on reactive oxygen species production and DNA damage in human SH-SY5Y neuroblastoma cells med./bio.

Aim of study (acc. to author)

Background/further details

Endpoint

• genotoxicity/mutation: DNA damage
• molecular biosynthesis: reactive oxygen species production

Exposure

• • RF field
• • CW continuous wave
  • PW pulsed wave
  • mobile communications
  • mobile phone
  • GSM

Extended view Compact view

Methods Endpoint/measurement parameters/methodology

• genotoxicity/mutation: DNA damage (alkaline comet assay)
• molecular biosynthesis: reactive oxygen species production (via a fluorescent probe 0, 5, 10, 15, 30 and 60 min after exposure)
• cell viability/cell division/proliferation: cell viability (propidium iodide stain)

Main outcome of study (acc. to author)

Study funded by

• Finnish Cultural Foundation of Northern Savo
• Finnish Graduate School of Environmental Health (SYTYKE)
• Finnish mobile phone manufacturers and operators
• Ministry of Education, Finland
• Tekes (National Technology Agency), Finland

Related articles

• Wang X et al. (2015): 8-oxoG DNA Glycosylase-1 Inhibition Sensitizes Neuro-2a Cells to Oxidative DNA Base Damage Induced by 900 MHz Radiofrequency Electromagnetic Radiation
• Kazemi E et al. (2015): Effect of 900 MHz Electromagnetic Radiation on the Induction of ROS in Human Peripheral Blood Mononuclear Cells
• Karaca E et al. (2012): The genotoxic effect of radiofrequency waves on mouse brain
• Poulletier de Gannes F et al. (2011): Effect of Exposure to the Edge Signal on Oxidative Stress in Brain Cell Models
• Perrin A et al. (2010): Evaluation of the co-genotoxic effects of 1800 MHz GSM radiofrequency exposure and a chemical mutagen in cultured human cells
• Kumar S et al. (2010): Evaluation of genotoxic effects in male Wistar rats following microwave exposure
• Xu S et al. (2010): Exposure to 1800 MHz radiofrequency radiation induces oxidative damage to mitochondrial DNA in primary cultured neurons
• Sannino A et al. (2009): Human fibroblasts and 900 MHz radiofrequency radiation: evaluation of DNA damage after exposure and co-exposure to 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5h)-furanone (MX)
• Brescia F et al. (2009): Reactive oxygen species formation is not enhanced by exposure to UMTS 1950 MHz radiation and co-exposure to ferrous ions in Jurkat cells
• Luukkonen J et al. (2009): Enhancement of chemically induced reactive oxygen species production and DNA damage in human SH-SY5Y neuroblastoma cells by 872 MHz radiofrequency radiation
• Verschaeve L (2009): Genetic damage in subjects exposed to radiofrequency radiation
• Benfante R et al. (2008): The expression of PHOX2A, PHOX2B and of their target gene dopamine-beta-hydroxylase (DbetaH) is not modified by exposure to extremely-low-frequency electromagnetic field (ELF-EMF) in a human neuronal model
• Tiwari R et al. (2008): Combinative Exposure Effect of Radio Frequency Signals from CDMA Mobile Phones and Aphidicolin on DNA Integrity
• Yao K et al. (2008): Electromagnetic noise inhibits radiofrequency radiation-induced DNA damage and reactive oxygen species increase in human lens epithelial cells
• Vijayalaxmi et al. (2008): Genetic damage in mammalian somatic cells exposed to radiofrequency radiation: a meta-analysis of data from 63 publications (1990-2005)
• Buttiglione M et al. (2007): Radiofrequency radiation (900 MHz) induces Egr-1 gene expression and affects cell-cycle control in human neuroblastoma cells
• Kanitz MH et al. (2007): Investigation of protein expression in magnetic field-treated human glioma cells
• Baohong W et al. (2007): Evaluating the combinative effects on human lymphocyte DNA damage induced by ultraviolet ray C plus 1.8 GHz microwaves using comet assay in vitro
• Verschaeve L et al. (2006): Investigation of co-genotoxic effects of radiofrequency electromagnetic fields in vivo
• Antonini RA et al. (2006): Extremely low-frequency electromagnetic field (ELF-EMF) does not affect the expression of alpha3, alpha5 and alpha7 nicotinic receptor subunit genes in SH-SY5Y neuroblastoma cell line
• Baohong W et al. (2005): Studying the synergistic damage effects induced by 1.8 GHz radiofrequency field radiation (RFR) with four chemical mutagens on human lymphocyte DNA using comet assay in vitro
• Zmyslony M et al. (2004): Acute exposure to 930 MHz CW electromagnetic radiation in vitro affects reactive oxygen species level in rat lymphocytes treated by iron ions
• Zhang MB et al. (2002): Study of low-intensity 2450-MHz microwave exposure enhancing the genotoxic effects of mitomycin C using micronucleus test and comet assay in vitro
• Maes A et al. (1996): 954 MHz microwaves enhance the mutagenic properties of mitomycin C
• Kerbacher JJ et al. (1990): Influence of radiofrequency radiation on chromosome aberrations in CHO cells and its interaction with DNA-damaging agents
• Meltz ML et al. (1989): Absence of mutagenic interaction between microwaves and mitomycin C in mammalian cells
• Ciaravino V et al. (1987): Effects of radiofrequency radiation and simultaneous exposure with mitomycin C on the frequency of sister chromatid exchanges in Chinese hamster ovary cells