Abnormality of synaptic vesicular associated proteins in cerebral cortex and hippocampus after microwave exposure med./bio.

To study the levels of different synaptic vesicular associated proteins (synapsin I, VAMP-2 (vesicle-associated membrane protein), syntaxin, and synaptophysin) in the synaptosomes of cerebral cortex and hippocampus after microwave exposure.

Among the synaptic vesicular associated proteins, synapsin I, VAMP, syntaxin and synaptophysin are believed to play an important role in the exocytosis.
25 rats were exposed and synaptosomes were prepared at six hours, one, three, and seven days after exposure.

• animal
• rat/Wistar
• whole body

Synapsin I was decreased in the cerebral cortex at three days after exposure. In the hippocampus synapsin I was increased at one day, decreased at three days, and increased again at seven days after exposure compared with the sham exposed controls.
Synaptophysin was increased in all samples (cerebral cortex and hippocampus) at one to seven days after exposure.
VAMP-2 was decreased at one and three days and syntaxin was decreased at six hours to three days after irradiation in the cerebral cortex and hippocampus.
The interactions between VAMP-2 and syntaxin were decreased at three to seven days after exposure in the cerebral cortex and hippocampus compared with the sham exposed controls.
These results suggest that 30 mW/cm² microwave exposure can result in the perturbation of the above-mentioned synaptic vesicles associated proteins.