To study the effects of continuous microwaves of different field intensities on acetylcholinesterase activity and protein conformation in muscle fractions from frog skeletal muscles.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
2.45 GHz
Modulation type:
CW
Exposure duration:
continuous for 30 min
|
|
|
Exposure 2:
2.45 GHz
Modulation type:
CW
Exposure duration:
continuous for 30 min
|
|
| Frequency | 2.45 GHz |
|---|---|
| Exposure duration | continuous for 30 min |
| Modulation type | CW |
|---|
| Exposure source |
|
|---|---|
| Sham exposure | A sham exposure was conducted. |
| Additional info | the petri dishes maintained at 1.8 and 2°C. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| SAR | 4.92 W/g | mean | calculated | - | - |
| power density | 100 W/m² | mean | measured | - | - |
Exposure to microwaves results in non-thermal, intensity-dependent, prolonged modification of acetylcholinesterase activity in frog skeletal muscles traced up to 48 hours after irradiation. A significant decrease in enzyme activity on the day of exposure was revealed. 48 hours later the decrease in enzyme activity in samples exposed to both high- and low-intensity microwaves was less than that in sham-exposed samples.
Infrared spectroscopy data argue for microwave-induced conformational changes in the secondary structure of muscle proteins: increased content of beta-structures, random coils, and amorphous structures, which were more expressed at irradiation with low field intensity.
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