Evaluation of genotoxic effects in human fibroblasts after intermittent exposure to 50 Hz electromagnetic fields: a confirmatory study med./bio.

[Bewertung genotoxischer Wirkungen in menschlichen Fibroblasten nach intermittierender Exposition bei elektromagnetischen 50 Hz-Feldern: Eine Bestätigungsstudie]

Ziel der Studie (lt. Autor)

Hintergrund/weitere Details

Endpunkt

• Genotoxizität/Mutation: DNA-Schaden (DNA-Einzelstrangbrüche)

Exposition/Befeldung (teilweise nur auf Englisch)

• • 50/60 Hz
  • magnetisches Feld
• • Hochspannungsfreileitung

Vollständige Ansicht Kompaktansicht

Methoden Endpunkt/Messparameter/Methodik

• Genotoxizität/Mutation: DNA-Schaden (alkalischer Comet-Assay)
• Zelllebensfähigkeit/Zellteilung/Zellproliferation: Zellzyklus-Kinetik (Mikronukleus-Assay); Zellproliferation (Proliferationsindex); zweikerniger Zell-Index

Hauptergebnis der Studie (lt. Autor)

Studie gefördert durch

• European Union (EU)/European Commission
• Ministero dell' Università e della Ricerca (MIUR (formerly MURST (Ministero dell' Università e della Ricerca Scientifica e Tecnologica); Ministry of University and Research), Italy
• VERUM Foundation (Stiftung für Verhalten und Umwelt), Germany

Replizierte Studien

• Ivancsits S et al. (2003): Intermittent extremely low frequency electromagnetic fields cause DNA damage in a dose-dependent way
• Ivancsits S et al. (2002): Induction of DNA strand breaks by intermittent exposure to extremely-low-frequency electromagnetic fields in human diploid fibroblasts

Themenverwandte Artikel

• Lv Y et al. (2021): Exposure to 50 Hz Extremely-Low-Frequency Magnetic Fields Induces No DNA Damage in Cells by Gamma H2AX Technology
• Sun C et al. (2018): Ataxia telangiectasia mutated deficiency does not result in genetic susceptibility to 50 Hz magnetic fields exposure in mouse embryonic fibroblasts
• Zhu K et al. (2016): Extremely low frequency magnetic fields do not induce DNA damage in human lens epithelial cells in vitro
• Kesari KK et al. (2015): Genomic instability induced by 50Hz magnetic fields is a dynamically evolving process not blocked by antioxidant treatment
• Jin YB et al. (2014): Absence of DNA damage after 60-Hz electromagnetic field exposure combined with ionizing radiation, hydrogen peroxide, or c-Myc overexpression
• Alcaraz M et al. (2014): Effect of long-term 50 Hz magnetic field exposure on the micronucleated polychromatic erythrocytes of mice
• Jin YB et al. (2012): Effects on micronuclei formation of 60-Hz electromagnetic field exposure with ionizing radiation, hydrogen peroxide, or c-Myc overexpression
• Focke F et al. (2010): DNA fragmentation in human fibroblasts under extremely low frequency electromagnetic field exposure
• Jian W et al. (2009): X-ray-induced apoptosis of BEL-7402 cell line enhanced by extremely low frequency electromagnetic field in vitro
• Burdak-Rothkamm S et al. (2009): DNA and chromosomal damage in response to intermittent extremely low-frequency magnetic fields
• Wahab MA et al. (2007): Elevated sister chromatid exchange frequencies in dividing human peripheral blood lymphocytes exposed to 50 Hz magnetic fields
• Williams PA et al. (2006): 14.6 mT ELF magnetic field exposure yields no DNA breaks in model system Salmonella, but provides evidence of heat stress protection
• Hone P et al. (2006): Chromatid damage in human lymphocytes is not affected by 50 Hz electromagnetic fields
• Villarini M et al. (2006): Effects of co-exposure to extremely low frequency (50 Hz) magnetic fields and xenobiotics determined in vitro by the alkaline comet assay
• Ivancsits S et al. (2005): Cell type-specific genotoxic effects of intermittent extremely low-frequency electromagnetic fields
• Koyama S et al. (2004): ELF electromagnetic fields increase hydrogen peroxide (H2O2)-induced mutations in pTN89 plasmids
• Pasquini R et al. (2003): Micronucleus induction in cells co-exposed in vitro to 50 Hz magnetic field and benzene, 1,4-benzenediol (hydroquinone) or 1,2,4-benzenetriol
• Ding GR et al. (2003): Induction of kinetochore-positive and kinetochore-negative micronuclei in CHO cells by ELF magnetic fields and/or X-rays
• Tokalov SV et al. (2003): The heat shock-induced cell cycle arrest is attenuated by weak electromagnetic fields
• Ivancsits S et al. (2003): Intermittent extremely low frequency electromagnetic fields cause DNA damage in a dose-dependent way
• Ivancsits S et al. (2002): Induction of DNA strand breaks by intermittent exposure to extremely-low-frequency electromagnetic fields in human diploid fibroblasts
• Harada S et al. (2001): Effects of high ELF magnetic fields on enzyme-catalyzed DNA and RNA synthesis in vitro and on a cell-free DNA mismatch repair
• Simko M et al. (2001): Micronucleus induction in Syrian hamster embryo cells following exposure to 50 Hz magnetic fields, benzo(a)pyrene, and TPA in vitro
• Scarfi MR et al. (1997): 50-Hz, 1-mT sinusoidal magnetic fields do not affect micronucleus frequency and cell proliferation in human lymphocytes from normal and Turner's syndrome subjects